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HIT-DH5a
 
 
 
Datasheets
 
 
   
 
 
General cloning, TA cloning, Blue/White screening
Recommended for cDNA library construction
Most popular cloning strain worldwide
 
 
 
 
Jumbo HIT-DH5a
2 x 1ml Competent cells
 
 
 
Value 108 HIT-DH5a
10 x 100µl Competent Cells
1 Control Plasmid
 
 
 
High 108 HIT-DH5a
10 x 100µl Competent Cells
1 Control Plasmid
 
 
 
Super 109 HIT-DH5a
10 x 100µl Competent Cells
1 Control Plasmid
 
 
 
Value 108 HIT-DH5a (80 vials Economy Package)
80 x 100µl Competent Cells
1 Control Plasmid
 
 
 
High 108 HIT-DH5a (80 vials Economy Package)
80 x 100µl Competent Cells
1 Control Plasmid
 
 
 
Super 109 HIT-DH5a (80 vials Economy Package)
80 x 100µl Competent Cells
1 Control Plasmid
 
 
 
 
 

HIT™-DH5a are unique chemically competent cells, which utilise a novel transformation protocol: High Ice Transformation. A simple ice incubation from 1-10 minutes is all that is required to transform the cells to high efficiency. There is no requirement for heat shock or recovery step.

Efficiency Levels
Each HIT™ shipment is -70°C electronically monitored and recorded for minimum efficiency guarantee.

HIT™ Value 108: DH5a
Minimum efficiency is 1 x 107 transformants/μg pUC19
Recommended for
-Subcloning
-General Cloning

HIT™ High 108:  DH5a
Minimum efficiency is 1 x 108 transformants/μg pUC19
-High efficiency cloning
-Cloning low abundance transcripts
-General Cloning
-Gene Library

HIT™ Super 109: DH5a
Minimum efficiency is 5 x 108 transformants/μg pUC19
-Maximum efficiency cloning
-Cloning low abundance transcripts
-Gene Library

Applications and Genotype
The HIT - DH5a strain allows blue-white color screening for recombinant plasmid and is an excellent host strain for routine cloning applications using plasmid or lambda vectors.

F-(80d lacZ M15) (lacZYA-argF) U169 hsdR17 (r- m+) recA1 endA1 relA1 deoR

Hit.jpg
 
 
Product Information
 
Cat.No. RH617-J
 
Jumbo HIT-DH5a
2 x 1ml Competent cells
 
 
Cat.No. RH617
 
Value 108 HIT-DH5a
10 x 100µl Competent Cells
1 Control Plasmid
 
 
Cat.No. RH618
 
High 108 HIT-DH5a
10 x 100µl Competent Cells
1 Control Plasmid
 
 
Cat.No. RH619
 
Super 109 HIT-DH5a
10 x 100µl Competent Cells
1 Control Plasmid
 
 
Cat.No. RH617-J80
 
Value 108 HIT-DH5a (80 vials Economy Package)
80 x 100µl Competent Cells
1 Control Plasmid
 
 
Cat.No. RH618-J80
 
High 108 HIT-DH5a (80 vials Economy Package)
80 x 100µl Competent Cells
1 Control Plasmid
 
 
Cat.No. RH619-J80
 
Super 109 HIT-DH5a (80 vials Economy Package)
80 x 100µl Competent Cells
1 Control Plasmid