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HIT-JM109
 
 
 
Datasheets
 
 
   
 
 
General cloning, TA cloning, Blue/White screening, Robotic screening.
Rapid growth results in pickable colonies within 8-10 hours
Recommended for gene library construction
 
 
 
 
Jumbo HIT-JM109
2 x 1ml Competent Cells
 
 
 
Value 108 HIT-JM109
10 x 100µl Competent Cells
1 Control Plasmid
 
 
 
High108 HIT-JM109
10 x 100µl Competent Cells
1 Control Plasmid
 
 
 
Value 108 HIT-JM109 (80 vials Economy Package)
80 x 100µl Competent Cells
1 Control Plasmid
 
 
 
High 108 HIT-JM109 (80 vials Economy Package)
80 x 100µl Competent Cells
1 Control Plasmid
 
 
 
 
 
HIT™-JM109 are unique chemically competent cells, which utilise a novel transformation protocol-High Ice Transformation. A simple ice incubation from 1-10 minutes is all that is required to transform the cells to high efficiency. There is no requirement for heat shock or recovery step.

Efficiency Levels
Each HIT™ shipment is -70°C electronically monitored and recorded for minimum efficiency guarantee.

HIT™ Value 108-JM109:
Minimum efficiency is 1 x 107 transformants/μg pUC19
Recommended for
-Subcloning
-General Cloning

HIT™ High 108-JM109:
Minimum efficiency is 1 x 108 transformants/μg pUC19
-High efficiency cloning
-Cloning low abundance transcripts
-General Cloning
-Gene Library

Applications and Genotype
The HIT™-JM109 strain allows blue-white color screening for recombinant lasmids and is an excellent host strain for routine cloning application using plasmid or lambda vectors.


Transform at 9am, pick at 5 pm!
Due to the rapid growth of this strain, HIT™ transformation and picking of colonies may be achieved in one day, potentially eliminating one day from the work schedule.


e14-(McrA-) recA1 endA1 gyrA96 thi-1 hsdR17(rK- mK+) supE44 relA (lac-proAB) [F' traD36 proAB lacIqZ M15]

Hit.jpg
 
 
Product Information
 
Cat.No. RH717-J
 
Jumbo HIT-JM109
2 x 1ml Competent Cells
 
 
Cat.No. RH717
 
Value 108 HIT-JM109
10 x 100µl Competent Cells
1 Control Plasmid
 
 
Cat.No. RH718
 
High108 HIT-JM109
10 x 100µl Competent Cells
1 Control Plasmid
 
 
Cat.No. RH717-J80
 
Value 108 HIT-JM109 (80 vials Economy Package)
80 x 100µl Competent Cells
1 Control Plasmid
 
 
Cat.No. RH718-J80
 
High 108 HIT-JM109 (80 vials Economy Package)
80 x 100µl Competent Cells
1 Control Plasmid