RBC Glass Plating Beads (4mm) provide an efficient and easy method to achieve optimal spreading on agar plates. The rolling action of the glass beads gently spreads the E.coli solution (or any bacterial/fungal solution) to reach areas of the plate that are inaccessible to traditional spreaders. Multiple plates containing beads and E.coli may be stacked and shaken simultaneously to increase spreading throughput and reduce cell stress. The beads may be recovered by washing with 70% ethanol, sterilized by autoclaving and oven dried.

ApplicationsIncreases Transformation Efficiency
Rapid Simultaneous Plating
Economical - 75-100 plates/bottle
Reusable
Sterile

Process
For regular plating of RBC HIT cells, 10 - 20 beads may be added immediately after pipetting 50-100ml of transformed cells onto each plate. Move the plate back and forth to roll the beads on the surface and change direction of shaking a few times to ensure even spreading. When the surface of the agar visibly begins to dry on the plate (20-40 seconds), pour off the beads and the plates are ready for incubation.