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	Total RNA Extraction Kit (Tissue)

		Protocols
		Datasheets
		MSDS

Sample Sources Mini/Maxi (Protocols Incl.):

Fresh/Frozen Animal Tissue, Blood, Cultured animal cells, Bacterial Cells

Sample Amount: 10-15 mg/100-200 mg

Yield: 3-25 μg/50-500 μg

Elution Volume: 50 μl/ 500 μl

*For certain protocols items such as DNase I, Lysozyme may need to be purchased separately

	Cat.No. YRT50
	50 mini preps/kit

	Cat.No. YRT100
	100 mini preps/kit

	Cat.No. YRBM25
	25 Maxi prep/kit

	Cat.No. YRBM10
	10 Maxi preps/kit


	The Total RNA Extraction Kit (Tissue) is specially designed for purification of total RNA from a variety of animal tissues or cells. The provided micropestle (Mini Format) efficiently homogenizes tissue samples in a microcentrifuge tube. The method uses detergents and a chaotropic salt to lyse cell and inactivate RNase. RNA in the chaotropic salt solution is bound to the glass fiber matrix of the column. Following washing off of the contaminants the purified RNA is eluted by RNase-free water. The entire procedure can be completed in 20-40 minutes. ssRNA and dsRNA of > 200 bps to 1000's of bps in length are efficiently purified. Applications -RT-PCR -northern blotting -primer extension -cDNA library construction Quality Control The quality of Total RNA Extraction Kit (Tissue) is tested on a lot-to-lot basis. Kits are tested by isolation of total RNA from 10mg mouse liver. More than 20μg of total RNA was quantified with a spectrophotometer and checked by formaldehyde agarose gel analysis. RT-PCR was used to ensure the quality of total RNA.

	Product Information
	Cat.No. YRT50
	50 mini preps/kit
	RB Buffer: 25ml W1 Buffer: 25ml Wash Buffer (concentrated): 12.5ml RNase-free Water: 6ml RB Column Set: 50 sets Filter Column Set: 50 sets Micropestles(RNase free): 50 pcs
	Cat.No. YRT100
	100 mini preps/kit
	RB Buffer: 50ml W1 Buffer: 50ml Wash Buffer (concentrated): 25ml RNase-free Water: 6ml RB Column Set: 100 sets Filter Column Set: 100 sets Micropestles(RNase free): 100pcs
	Cat.No. YRBM25
	25 Maxi prep/kit
	Combined Blood-Cells-Tissue Format RBC Lysis Buffer: 500 x 2 ml RB Buffer: 120ml W1 Buffer: 130ml Wash Buffer (concentrated) : 50ml RNase-free water: 30ml Filter Maxi Column Set: 25 sets RB Maxi Column Set: 25 sets
	Cat.No. YRBM10
	10 Maxi preps/kit
	Combined Blood-Cells-Tissue Format RBC Lysis Buffer: 125+500 ml RB Buffer: 60ml W1 Buffer: 50ml Wash Buffer (concentrated) : 25ml RNase-free water: 6ml Filter Maxi Column Set: 10 sets RB Maxi Column Set: 10 sets