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Total RNA Extraction Kit (Plant)
 
 
 
Datasheets
 
MSDS
 
   
 
 
Sample Sources: Mini/Maxi: Fresh/Frozen Leaves, Fresh/Frozen Tissue
Sample Amount: 50mg/500mg
Yield:  5-30μg/50-300μg
Elution Volume: 50ml/ 500ml

*For certain protocols items such as DNase I, Lysozyme may need to be purchased separately

 
 
 
 
50 Mini preps/kit
 
 
 
100 Mini preps/kit
 
 
 
10 Maxi preps/kit
 
 
 
25 Maxi preps/kit
 
 
 
 
 

The Total RNA Extraction Kit (Plant) is specially designed for purification of total RNA from a variety of plant tissues. The provided micropestle efficiently homogenizes tissue samples in a microcentrifuge tube. The method uses detergents and a chaotropic salt to lyse cell and inactivate RNase. RNA in the chaotropic salt solution is bound to the glass fiber matrix of the column. Following washing off of the contaminants the purified RNA is eluted by RNase-free water. The entire procedure can be completed in 30-40 minutes. ssRNA and dsRNA of > 200 bps to 1000's of bps in length are efficiently purified.

Applications
-RT-PCR
-northern blotting
-primer extension
-cDNA library construction
-Microarray hybridization (cDNA)

Quality Control
The quality of Total RNA Extraction Kit (Tissue) is tested on a lot-to-lot basis.

Total_RNA_Extraction_Kit_Plant_YRP50_100.jpg

 
 
Product Information
 
Cat.No. YRP50
 
50 Mini preps/kit
 
RB Buffer: 30ml
PRB Buffer: 30ml  
W1 Buffer: 25ml
Wash Buffer (concentrated): 12.5ml
RNase-free Water:  6ml 
Filter Column Set:50 sets 
RB Column Set: 50 sets
 
Cat.No. YRP100
 
100 Mini preps/kit
 
RB Buffer:  60ml
PRB Buffer: 60ml  
W1 Buffer: 50ml
Wash Buffer (concentrated):  25ml
RNase-free Water :  6ml
Filter Column Set: 100 sets
RB Column Set: 100 sets
 
Cat.No. YRPM10
 
10 Maxi preps/kit
 
RB Buffer: 60ml
PRB Buffer: 60ml
W1 Buffer: 50ml
Wash Buffer (concentrated): 12.5ml
RNase-free Water: 6ml
Filter Maxi Column Set: 10 sets
RB Maxi Column Set: 10 sets
 
Cat.No. YRPM25
 
25 Maxi preps/kit
 
RB Buffer: 150ml
PRB Buffer: 150ml
W1 Buffer: 130ml
Wash Buffer (concentrated): 50ml
RNase-free Water :   30ml
Filter Maxi Column Set: 25 sets
RB Maxi Column Set: 25 sets