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	Total RNA Extraction kit (Blood/Bacteria/Cultured Cells)

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		Datasheets
		MSDS

Sample source : Animal Tissues, Whole Blood/ Buffy Coat, Cultured Animal Cells, Gram -ve/+ve Bacteria
Sample size : Mini- 10 mg Tissue, 10⁷Animal Cells, 300μl Whole Blood,10⁹Bacterial Cells

Maxi- 100-200mg animal tissue, 10⁷-10⁸cultured cell, 5ml blood sample

Preparation time: 20 min/ 60 mins

Yield: Mini- 2-8µg Maxi- < 500µg

	Cat.No. YRB50
	50 mini preps / kit

	Cat.No. YRB100
	100 mini preps / kit

	Cat.No. YRBM10
	10 maxi preps / kit

	Cat.No. YRBM25
	25 maxi preps / kit


	The Total RNA Extraction Kit (Blood/Bacterial/Cultured Cells) is specially designed for purification of total RNA from bacterial, cultured cells and fresh human whole blood. The method uses detergents and a chaotropic salt to lyse the cells and inactivate RNase. The lysate is applied to provided Lysate Filter columns (Mini System) to remove cell debris and ensure complete cell lysis. RNA in chaotropic salt solutions binds to the glass fiber matrix of the columns. Following washing off of contaminants, the purified RNA is eluted by RNase-free water. ssRNA and dsRNA of > 200 bps to 1000's of bps in length are efficiently purified. Applications RT-PCR, cDNA synthesis, In Vitro Translation, Primer Extension, Northern blotting, Micorarray prep Quality Control The quality of Total RNA Extraction Kit (Blood/Cells) is tested on a lot-to-lot basis. The kits are tested by isolation of total RNA from 300μl of fresh human whole blood. More than 1μg of total RNA was quantified with a spectrophotometer and checked by formaldehyde agarose gel analysis. Finally, RT-PCR was used to ensure the quality of total RNA.

	Product Information
	Cat.No. YRB50
	50 mini preps / kit
	RBC Lysis Buffer: 120 ml RB Buffer: 30ml RT Buffer: 15 ml W1 Buffer: 25 ml Wash Buffer (concentrated): 12.5 ml RNase-free Water: 6ml RB Column Set: 50 sets Filter Column Set: 50 sets All components are RNase -Free
	Cat.No. YRB100
	100 mini preps / kit
	RBC Lysis Buffer: 240ml RB Buffer: 60ml RT Buffer: 30ml W1 Buffer: 50ml Wash Buffer (concentrated): 25ml RNase-free Water: 30ml RB Column Set: 100 sets Filter Column Set: 100 sets All components are RNase -Free
	Cat.No. YRBM10
	10 maxi preps / kit
	RBC Lysis Buffer: 125+250 ml RB Buffer: 60 ml W1 Buffer: 50 ml Wash Buffer (concentrated): 25 ml RNase-free Water: 6 ml RB Maxi Column Set: 10 sets Filter Maxi Column Set: 10 sets All components are RNase -Free
	Cat.No. YRBM25
	25 maxi preps / kit
	RBC Lysis Buffer: 500 x2 ml RB Buffer: 150 ml W1 Buffer: 130 ml Wash Buffer (concentrated): 50 ml RNase-free Water: 30 ml RB Maxi Column Set: 25 sets Filter Maxi Column Set: 25 sets All components are RNase -Free