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Genomic DNA Extraction Kit (Blood/Bacteria/Cultured Cells)
 
 
 
Datasheets
 
 
   
 
 
Genomic DNA MIni Kit
Sample: 300µl Whole Blood, 107 Cultured Cells,
                109 Bacteria (Gram +/-), 10Yeast
Average Yields: 200µl  Whole Blood -6µg, 200µl
     Buffy-50µg, 5 x 106Lymph./Cult. Cells -50µg 

Genomic DNA Maxi Kits
Sample (Protocols Included): Fresh/ Frozen Blood
Average Yields:  140/75µg
 
 
 
 
50 mini preps/kit
 
 
 
100 mini preps/kit
 
 
 
10 maxi preps/kit
 
 
 
25 Maxi preps/kit
 
 
 
 
 

Genomic DNA Extraction Kits (Blood/Bacteria/Cultured Cells) provides a fast and economical method for purification of total DNA (including genomic, mitochondrial and viral DNA) from whole blood (fresh/frozen), plasma, serum, buffy coat, other body fluids, lymphocytes and cultured cells. This kit is also ideal for bacterial and cultured cells. The blood protocol utilises RBC lysis buffer, GB buffer and a rapid heating step to release DNA into solution. DNA in the chaotropic salt solution binds to the glass fiber matrix of column. After washing off the contaminants, the purified DNA is eluted by low salt elution buffer or water. There is no requirement for phenol/chloroform extraction or alcohol precipitation. Purified DNA of approximately 20-30 kb is suitable for PCR or other enzymatic reactions.

Applications
-PCR
-Southern blotting
-PADP/ AFLP

Product Intended Use (Research/Clinical Application)*

Genomic_DNA_Extraction_Kit_Blood_Bacteria_Cultured_Cells_YGB100_300.jpg

 
 
Product Information
 
Cat.No. YGB50
 
50 mini preps/kit
 
50  mini preps / kit
RBC Lysis Buffer: 100ml
GB Buffer:  15ml
GT Buffer: 15ml
W1 Buffer : 25ml
Wash Buffer (Concentrated): 12.5ml
Elution Buffer :  30 ml
Proteinase K :  11 mg   
GD Columns:  50 pcs
2 ml Collection Tubes:  50 pcs

 
Cat.No. YGB100
 
100 mini preps/kit
 
100 mini preps / kit
RBC Lysis Buffer: 200ml
GB Buffer: 30ml
GT Buffer: 30ml
W1 Buffer : 50ml
Wash Buffer (Concentrated): 25ml
Elution Buffer :  30ml
Proteinase K :  11 mg  x 2       
GD Columns:  100 pcs
2 ml Collection Tubes:  100 pcs
 
Cat.No. YGBM10
 
10 maxi preps/kit
 
10 maxi preps / kit
RBC Lysis Buffer: 125ml & 250ml
GB Buffer: 120ml
W1 Buffer : 50ml
Wash Buffer (Concentrated): 25 ml 
Elution Buffer :  50 ml       
GD Maxi Columns:  10 pcs
 
Cat.No. YGBM25
 
25 Maxi preps/kit
 
25 maxi preps / kit
RBC Lysis Buffer: 500ml  x 2
GB Buffer: 240ml
W1 Buffer : 130ml
Wash Buffer (Concentrated): 50ml
Elution Buffer :  120ml       
GD  Maxi Columns:  25 pcs