The Plant Genomic DNA Extraction Kit (Duo Buffer System) provides a fast and simple method to isolate DNA from plant tissue and cells. In the first step the sample is lysed by homogenization. The lysate is treated with RNase A to remove RNA. In the presence of a chaotropic salt, the genomic DNA in the lysate binds to the glass fiber matrix in the spin column. The contaminants are washed with an ethanol based wash buffer and purified genomic DNA is eluted by low salt elution buffer or water. The protocol does not require DNA phenol extraction and alcohol precipitation. The entire procedure can be completed in 60 minutes.
Applications
PCR/ Real-time PCR, Southern blotting, RADP/ AFLP
Quality Control
The quality of the Plant Genomic DNA Mini Kit is tested on a lot-to-lot basis. The kits are tested by isolation of genomic DNA from 100mg young leaves.