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Genomic DNA Extraction Kit (Plant)
 
 
 
Datasheets
 
 
   
 
 
Plant Genomic DNA Extraction  Mini/ Maxi Kit
Sample: 100mg /  1g of plant tissue
Yield: 20µg/  300µg
Operation time: < 60 mins
Elution Volume: 50/1000µl
 
 
 
 
50 Mini preps/kit
 
 
 
100 Mini preps/kit
 
 
 
10 Maxi preps/kit
 
 
 
25 Maxi preps/kit
 
 
 
 
 

The Plant Genomic DNA Extraction Kit (Duo Buffer System) provides a fast and simple method to isolate DNA from plant tissue and cells. In the first step the sample is lysed by homogenization. The lysate is treated with RNase A to remove RNA. In the presence of a chaotropic salt, the genomic DNA in the lysate binds to the glass fiber matrix in the spin column. The contaminants are washed with an ethanol based wash buffer and purified genomic DNA is eluted by low salt elution buffer or water. The protocol does not require DNA phenol extraction and alcohol precipitation. The entire procedure can be completed in 60 minutes.

Applications
PCR/ Real-time PCR, Southern blotting, RADP/ AFLP

Quality Control
The quality of the Plant Genomic DNA Mini Kit is tested on a lot-to-lot basis. The kits are tested by isolation of genomic DNA from 100mg young leaves.

 
 
Product Information
 
Cat.No. YGP50
 
50 Mini preps/kit
 
50 mini preps / kit
GP1 Buffer: 25ml
GPX1 Buffer: 25ml
GP2 Buffer: 6ml
GP3 Buffer(concentrated): 15ml
W1 Buffer: 25ml
Wash Buffer (concentrated): 12.5ml
Elution Buffer : 30ml
RNase A(10mg/ml): 275µl
Filter Column: 50 pcs
GD Columns: 50 pcs
2 ml Collection Tube: 100 pcs
 
Cat.No. YGP100
 
100 Mini preps/kit
 
100 mini preps / kit
GP1 Buffer: 50ml
GPX1 Buffer: 50ml
GP2 Buffer: 15ml
GP3 Buffer (concentrated): 30ml
W1 Buffer: 50ml
Wash Buffer (concentrated): 25ml
Elution Buffer : 30ml
RNase A(10mg/ml): 550µl
GD Columns: 100 pcs
Filter Column: 100pcs
2 ml Collection: 200 pcs
 
Cat.No. YGPM10
 
10 Maxi preps/kit
 
10  maxi preps / kit
GP1 Buffer: 50ml
GPX1 Buffer: 50ml
GP2 Buffer: 15ml
GP3 Buffer: 30ml
W1 Buffer : 50ml
Wash Buffer (conc.): 25ml
Elution Buffer : 30ml
RNase A (10 mg/ ml):  550μl
Maxi Filter Column: 10 pcs
GD Maxi Column: 10 pcs

 
Cat.No. YGPM25
 
25 Maxi preps/kit
 
25 maxi preps / kit
GP1 Buffer: 125ml
GPX1 Buffer: 125ml
GP2 Buffer: 30ml
GBP3 Buffer: 70ml
W1 Buffer : 130ml
Wash Buffer (conc.): 50ml
Elution Buffer : 60ml
RNase A (10 mg/ ml):  650μl  x 2
Maxi Filter Column: 25 pcs
GD Maxi Column: 25 pcs