Applications
For general laboratory use. The purified total nucleic acid is suitable for highly sensitive and quantitative PCR. MagCore Viral Nucleic Acid Extraction Kit has been performed and proved with HBV, HCV, HIV and influenza viruses as downstream applications.
Advantage
- High performance with downstream applications such as qPCR.
- Directly use in downstream studies such as PCR and Restriction Digestion.
- Pre-filled cartridge with all reagents independently sealed prevents contamination.
- Disposable cartridges and pipette tips per extraction prevents cross contamination.
- True walk-away extraction system.
- UV light and stainless steel surfaces for easy cleaning.
MagCore® Viral Nucleic Acid Extraction Kit Real-Time PCR testing
As can be seen in the accompanying curve, RBC’s MagCore® Viral Nucleic Acid Extraction System provides sufficient purified viral nucleic acid for sensitive downstream detection systems such as Real-Time PCR.
HBV detection by Real-Time PCR
RBC MagCore® Viral Nucleic Acid Extraction Kit was used to purify serum containing different concentrations of HBV. The HBV nucleic acid was subsequently detected by Real-Time PCR.
Figure 1. HBV sensitive detection by Nested PCR
Used RBC MagCore® Viral Nucleic Acid Extraction kit to purify serum contains different concentration HBV, and then detected HBV by Nested PCR.
Lane 1, 100 HBV serums. Lane 2, 101 HBV serums.
Lane 3, 102 HBV serums. Lane 4, 103 HBV serums.
Lane N, Negative control.
Lane M, RBC 100 bp DNA Ladder Marker.
Figure 2. HCV sensitive detection by Nested PCR
Used RBC MagCore® Viral Nucleic Acid Extraction kit to purify serum contains different concentration HCV, and then detected HCV by Nested PCR.
Lane 1, 5X100 HCV serums. Lane 2, 5X101 HCV serums.
Lane 3, 5X102 HCV serums. Lane 4, 5X103 HCV serums.
Lane 5, 5X104 HCV serums. Lane 6, 5X105 HCV serums.
Lane P, Positive control.
Lane N, Negative control.
Lane M, RBC 100 bp DNA Ladder Marker.
