RBC Polymerases Introduction
RBC Bioscience provides a wide selection of extremely economical and high quality DNA Polymerases. These recombinant polymerases boast superior thermostable properties and are quality tested at every step of purification and packaging. Choose from the table below for a polymerase most suitable for your application. If none are suitable inquire about RBC Bioscience’s custom solutions for polymerases. RBC Taq Polymerases are available at very economical pricing yet offer superior results compared to most competitors.
- 2X SYBR Green Premix contains:
- RBC SensiZyme™ HotStart Taq DNA polymerase.
- SYBR Green real-time PCR Buffer.
ThermOne™ Real-Time Premix (SYBR Green) already contains SYBR Green I and can be used for QPCR with a fluorescent intercalator displacement assay and is suitable for LightCycler® by ROCHE.
Diagnostics, ABI PRISM® 7700 by Applied Biosystems, iCycler® by BioRad, Rotor-Gene® by Corbett and others
SYBR Green Premix Performance Test
Consistently high specificity over a broad dynamic range. Ten fold serial dilution (10-2~10-9 ) of plasmid DNA were amplified using primers specific to the NNV gene. Triplicate reactions at each concentration were amplified along with no-template controls. Standard curve had r=0.999, efficiency=92.1%, Standard deviation of Ct < 1.0.
Compare ThermOne SYBG Real-Time Premix (left) with Roche (right) by human Beta-actin (220 bp) amplification and the testing concentrations from 1ug to 1 pg.