

By using Agilent 2100 Bioanalyzer to quantify and qualify the RNA that extracted from three different kind of cultured cells. Compared to other purification method Q in the market, the MagCore Total RNA cultured cells Kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA). The purified RNA is ready for Northern analysis, RT-PCR, MicroArray and real-time PCR
Agilent 2100 Bioanalyzer analysis
|
Sample name |
Sample volume (μl) |
RNA conc. (ng/μl) |
RNA quantity (ng) |
OD260/280 Ratio |
Bioanalyzer chip lane location |
28S/18S Ratio |
RIN |
|
|---|---|---|---|---|---|---|---|---|
| #1 | C1 Q | 175 μl | 69.72 | 11852.4 | 2.07 | 1 | 2.1 | 9.8 |
| #2 | C1 | 175 μl | 84.98 | 14446.6 | 2.03 | 2 | 1.4 | 9 |
| #3 | T1 Q | 175 μl | 73.05 | 12418.5 | 2 | 3 | 2.1 | 10 |
| #4 | T1 | 175 μl | 72.58 | 12338.6 | 2 | 4 | 1.8 | 9.2 |
| #5 | H1 Q | 175 μl | 177.19 | 30122.3 | 2.05 | 5 | 1.9 | 10 |
| #6 | H1 | 175 μl | 194.62 | 33085.4 | 2.06 | 6 | 1.7 | 9.2 |

