MagCore® Genomic DNA Microbial Library-Ready Kit is specially designed for isolating high-quality microbial genomic DNA from bacteria, yeast, and fungi. This kit efficiently isolates total DNA from tough-to-lyse Gram-positive and Gram-negative bacteria, as well as yeast and fungi, by overcoming cell wall resistance through effective lysis and homogenization using Bead-Beating Tubes and optimized lysis chemistry. The hands-on time can be completed in as little as 10–25 minutes. In addition, inhibitory substances (e.g., polysaccharides and proteins) commonly present in microbial samples are efficiently removed through a one-step bead-beating process followed by fully automated purification. The purified DNA is ready for downstream molecular applications, including Nanopore sequencing, NGS, qPCR, and 16S rRNA gene sequencing.