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Home Products MagCore Kits Genomic DNA MagCore® Cultured cells DNA Kit
110
Cat.No.:
  • MCC-01
  • MCC-02

MagCore® Cultured cells DNA Kit

For genomic DNA extraction from cultured cells and amniotic fluid

MagCore® Cultured Cells DNA Kit is designed to extract genomic DNA from up to 5x106 cultured cells using MagCore® automated extraction systems. The kit contains all required reagents and labware for automated extraction using magnetic-particle technology. Reagents are supplied in prefilled cartridges, which can be easily loaded into the MagCore®  instrument.

MagCore® Cultured cells DNA Kit

Feature

High performance of purified DNA in downstream applications such as qPCR.

Efficient isolation of DNA from up to 5x106 cells.

Cartridges are pre-filled and sealed to prevent contamination.

No phenol or chloroform extraction.

Efficient removal of contaminants and inhibitors.

Applications

High-quality DNA available for downstream applications, including:

  1. PCR and real-time PCR
  2. Next Generation Sequencing (NGS)

Performance

DNA quality analysis by Spectrophotometer

DNA of three different cultured cells was extracted by the employment of
MagCore® HF16 and MagCore® Cultured Cells DNA Kit (200μl sample volume).
The following data show the yield and purity of DNA of three samples.

Sample 1: COS 7 (1.2x107)
Sample 2: Junkat (1x106)
Sample 3: HEK293 (1x106)

  1 2 3
Yield (mg) 16.44 13.08 24.42
DNA purity (A260/280) 1.93 1.87 1.92


Amniotic fluid DNA extraction

Result
DNA quality is checked by the NanoDrop spectrophotometer and agarose gel electrophoresis after DNA purification from amniotic-fluid cells. Cells were harvested from 10~15 ml amniotic fluid samples at pregnancy weeks 16-18 by centrifugation for 10 minutes at 3000 rpm.

Sample ID Conc. (pg/μl) A 260 / 280 A 260 / 230 Yield (μg)
(1) GPT2967P 22.68 1.84 1.07 1.36
(2) GPT2952P 33.98 1.92 1.57 2.04
(3) NP679P 33.65 1.80 1.34 2.02
(4) NP777P 39.22 1.86 1.20 2.35
(5) MP795P 22.05 1.79 0.97 1.32

Specification

Cartridge Contents

Cartridge Contents
  • Cartridge Contents
  • Cartridge Contents
  • Cartridge Contents
  • Cartridge Contents
  • Cartridge Contents
  • Cartridge Contents
  • Cartridge Contents
  • 1

    Heat block Well 1

  • 2

    Heat block Well 2

  • 3

    Empty

  • 4

    Empty

  • 5

    Elution Buffer 1000µl

  • 6

    ddH2O 1000µl

  • 7

    Wash 2 Buffer 1000µl

  • 8

    Wash 1 Buffer 1000μl

  • 9

    Beads Mixture 500μl

  • 10

    Binding Buffer 1000µl

  • 11

    Lysis Buffer 500µl

  • 12

    Empty

  • 13

    Magnetic Beads Separation Well 1

  • 14

    Magnetic Beads Separation Well 2

Cat.No. : MCC-01

introduction

Storage And Stability : Store at Room Temperature

Packing : 36 tests / Kit

Contents

Pre-Filled Cartridge Reagent ( 36 pcs )

Pipette Tip and Holder Set ( 36 sets )

Sample Tube ( 36 pcs )

Elution Tube ( 36 pcs )

Proteinase K ( 11 mg/tube x 1 pcs )

PK Storage Buffer ( 1.25 ml/tube x 1 pcs )

Cat.No. : MCC-01

Cat.No. : MCC-02

introduction

Storage And Stability : Store at Room Temperature

Packing : 96 tests / Kit

Contents

Pre-Filled Cartridge Reagent ( 96 pcs )

Pipette Tip and Holder Set ( 96 sets )

Sample Tube ( 96 pcs )

Elution Tube ( 96 pcs )

Proteinase K ( 11 mg/tube x 2 pcs )

PK Storage Buffer ( 1.25 ml/tube x 2 pcs )

Cat.No. : MCC-02

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