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Home Products MagCore Kits Genomic DNA MagCore® Circulating DNA large volume kit 4ml
115
Cat.No.:
  • MPD4000-01
  • MPD4000-03

MagCore® Circulating DNA large volume kit 4ml

For extraction of free circulating DNA from human plasma or serum

Applicable Models : EDAPlus IISuperHF16HF16 Plus

MagCore® Circulating DNA large volume kit is designed to purify free circulating DNA from human serum or plasma using MagCore® automated extraction systems. The kit contains all required reagents and labware for automated purification based on magnetic-particle technology. Reagents necessary for a complete process are supplied and pre-filled in the cartridges, which can be easily loaded into the MagCore® instrument.

MagCore® Circulating DNA large volume kit 4ml

Feature

High performance of purified DNA in downstream applications such as qPCR.

Efficient recovery of fragmented DNA.

Pre-filled and sealed buffer cartridges prevent contamination.

No phenol or chloroform extraction.

Efficient removal of contaminants and inhibitors.

Applications

The MagCore® Plasma DNA Extraction Kit efficiently purifies free circulating DNA.
Sample types include:

  1. Human plasma
  2. Human serum

High-quality DNA available for downstream applications, including:

  1. PCR and real-time PCR
  2. Next Generation Sequencing (NGS)

Performance

High stability of free DNA

Quality and quantity analysis of the free DNA by Agilent 2100 Bioanalyzer.
It is shown by the evidence that free DNA of high quality and stability is possible to extract from 5 different healthy  plasma samples by using MagCore products.

Sample
ID		 Sample
type		 Plasma
(ml)		 Qubit
(ng/μl)		 Ct value
(GAPDH gene)		 Bioanalyzer
Conc.
(pg/μl)		 35 bp
marker
Conc.
(pg/μl)		 10380 bp marker
Conc.
(pg/μl)
1 Human
plasma		 4 0.262 18.52 127.21 125 75
2 Human
plasma		 4 0.25 18.59 124.62 125 75
3 Human
plasma		 4 0.22 18.78 111.11 125 75
4 Human
lasma		 4 0.29 18.45 153.24 125 75
5 Human
plasma		 4 0.278 18.49 130.97 125 75


Comparison of free DNA from 1.2 ml and 4 ml plasma

Quality and quantity analysis of the free DNA is performed using the Agilent Bioanalyzer 2100. 
Superior quality DNA is available from plasma samples through MagCore products.

Sample
ID		 Kits
Code		 Sample
type		 Plasma
(ml)		 Qubit
(ng/μl)		 Ct value
(GAPDH gene)		  Bioanalyzer 
Conc.
(pg/μl)		 35 bp
marker
Conc.
(pg/μl)		 10380 bp
marker
Conc.
(pg/μl)
1 105 Human
plasma		 4 0.262 18.52 127.21 125 75
2 105 Human
plasma		 4 0.25 18.59 124.62 125 75
3 115 Human
plasma		 4 0.22 18.78 111.11 125 75
4 115 Human
plasma		 4 0.29 18.45 153.24 125 75

Specification

Cartridge Contents

Cartridge Contents
  • Cartridge Contents
  • Cartridge Contents
  • Cartridge Contents
  • Cartridge Contents
  • Cartridge Contents
  • Cartridge Contents
  • Cartridge Contents
  • Cartridge Contents
  • Cartridge Contents
  • 1

    Heat block Well 1

  • 2

    Heat block Well 2

  • 3

    Empty

  • 4

    Lysis Buffer 1000µl

  • 5

    RNase Free Water 1000µl

  • 6

    RNase Free Water 1000µl

  • 7

    Wash 2 Buffer 1000µl

  • 8

    Wash 1 Buffer 1000μl

  • 9

    Beads Mixture 500μl

  • 10

    Binding Buffer 1800µl

  • 11

    Lysis Buffer 1500µl

  • 12

    Binding Buffer 1800µl

  • 13

    Magnetic Beads Separation Well 1

  • 14

    Magnetic Beads Separation Well 2

Cat.No. : MPD4000-01

introduction

Storage And Stability : Store at Room Temperature

Packing : 24 tests / Kit

Contents

Pre-Filled Cartridge Reagent ( 24 pcs )

Pipette Tip and Holder Set ( 24 sets )

Sample Tube ( 5ml/tube x 24 pcs )

Elution Tube ( 24 pcs )

Proteinase K ( 11 mg/tube x 3 pcs )

PK Storage Buffer ( 1.25 ml/tube x 3 pcs )

Cat.No. : MPD4000-01

Cat.No. : MPD4000-03

introduction

Storage And Stability : Store at Room Temperature

Packing : 96 tests / Kit

Contents

Pre-Filled Cartridge Reagent ( 96 pcs )

Pipette Tip and Holder Set ( 96 sets )

Sample Tube ( 5ml/tube x 96 pcs )

Elution Tube ( 96 pcs )

Proteinase K ( 11 mg/tube x 10 pcs )

PK Storage Buffer ( 1.25 ml/tube x 10 pcs )

Cat.No. : MPD4000-03

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