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Home Products MagCore Kits Viral Nucleic Acids MagCore® Viral Nucleic Acid Extraction Kit Low PCR Inhibition
202
Cat.No.:
  • MVN400-03
  • MVN400-04

MagCore® Viral Nucleic Acid Extraction Kit Low PCR Inhibition

For extraction of viral DNA/RNA from serum plasma and cell-free body fluids

The MagCore® Viral Nucleic Acid Extraction Kit is designed for purifying viral DNA and RNA from serum, plasma, and cell-free body fluids.MagCore® magnetic particle technology delivers high-quality DNA/RNA that is suitable for direct use in downstream applications such as amplification or other enzymatic reactions. To minimize the risk of cross-contamination, plastic consumables inside the Kit are DNase/RNase treated and the operation system is designed to individually process samples at the same time. Multiple protocols are installed on the instrument and optimized for different sample volumes.

MagCore® Viral Nucleic Acid Extraction Kit Low PCR Inhibition

Feature

High performance of purified DNA in downstream applications such as qPCR.

High analytical sensitivity.

Cartridges are pre-filled and sealed to prevent contamination.

Walkaway processing improves work efficiency.

Applications

MagCore® Viral Nucleic Acid Extraction Kit is designed to allow automated processing of multiple sample types in the same run.
Sample types include:

  1. Cell-free body fluids
  2. Plasma and serum
  3. CSF
  4. Urine

Performance

HBV detection by Real-Time PCR
The isolations of HBV nucleic acid from samples containing different concentrations of HBV were subsequently detected by Real-Time PCR

Sensitivity test for HBV detection

Lane 1 1x100 HBV serums.
Lane 2 1x101 HBV serums.
Lane 3 1x102 HBV serums.
Lane 4 1x103 HBV serums.
Lane N Negative control.
Lane M RBC 100 bp DNA Ladder Marker.



Figure 1. Nested PCR results of HBV at different concentrations.
Viral nucleic acids were purified from samples containing different amounts of HBV using MagCore® Viral Nucleic Acid Extraction Kit.

Sensitivity test for HCV detection

Lane 1 5x100 HCV serums.
Lane 2 5x101 HCV serums.
Lane 3 5x102 HCV serums.
Lane 4 5x103 HCV serums.
Lane 5 5x104 HCV serums.
Lane 6 5x105 HCV serums.
Lane P Positive control.
Lane N Negative control.
Lane M RBC 100 bp DNA Ladder Marker.


Figure 2. Nested PCR results of HCV at different concentrations.
Viral nucleic acids were purified from samples containing different amount of HCV using MagCore® Viral Nucleic Acid Extraction Kit.

Specification

Cartridge Contents

Cartridge Contents
  • Cartridge Contents
  • Cartridge Contents
  • Cartridge Contents
  • Cartridge Contents
  • Cartridge Contents
  • Cartridge Contents
  • Cartridge Contents
  • Cartridge Contents
  • Cartridge Contents
  • 1

    Heat block Well 1

  • 2

    Heat block Well 2

  • 3

    Wash 2 Buffer 1000µl

  • 4

    Wash 2 Buffer 1000µl

  • 5

    RNase Free Water 1000µl

  • 6

    RNase Free Water 1000µl

  • 7

    Wash 1 Buffer 1000µl

  • 8

    Wash 1 Buffer 1000μl

  • 9

    Beads Mixture 500μl

  • 10

    Binding Buffer 1000µl

  • 11

    Lysis Buffer 1000µl

  • 12

    Empty

  • 13

    Magnetic Beads Separation Well 1

  • 14

    Magnetic Beads Separation Well 2

Cat.No. : MVN400-03

introduction

Storage And Stability : Store at Room Temperature

Packing : 36 tests / Kit

Contents

Pre-Filled Cartridge Reagent ( 36 pcs )

Pipette Tip and Holder Set ( 36 sets )

Sample Tube ( 36 pcs )

Elution Tube ( 36 pcs )

Proteinase K ( 11 mg/tube x 1 pcs )

PK Storage Buffer ( 1.25 ml/tube x 1 pcs )

Carrier RNA ( 1 mg/pcs x 1 pcs )

RNase Free Water ( 1.25 ml/tube x 1 pcs )

Cat.No. : MVN400-03

Cat.No. : MVN400-04

introduction

Storage And Stability : Store at Room Temperature

Packing : 96 tests / Kit

Contents

Pre-Filled Cartridge Reagent ( 96 pcs )

Pipette Tip and Holder Set ( 96 sets )

Sample Tube ( 5ml/tube x 96 pcs )

Elution Tube ( 96 pcs )

Carrier RNA ( 1 mg/pcs x 1 pcs )

RNase Free Water ( 1.25 ml/tube x 1 pcs )

Proteinase K ( 11 mg/tube x 2 pcs )

PK Storage Buffer ( 1.25 ml/tube x 2 pcs )

Cat.No. : MVN400-04

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