Language

Products

About us

Newsroom

Resources

Investors

Contact us
Privacy
Newsroom
Home Products MagCore Kits Genomic DNA MagCore® Gut Microbiome DNA Kit
504
Cat.No.:
  • MGM-01
  • MGM-02

MagCore® Gut Microbiome DNA Kit

For extraction of microbial and host genomic DNA from stool samples

MagCore® Gut Microbiome DNA Kit is designed to isolate high-quality microbial and host genomic DNA from stool and gut samples. With a short pretreatment, DNA from tough-to-lyse Gram-positive and Gram-negative bacteria that inhabit fecal samples can be successfully isolated with efficient lysis and homogenized with Beads-Beating Tube. In addition, inhibitor substances (e.g. polysaccharides, protein, etc.) commonly found in stools are removed with a one-step IR Buffer. The crude DNA is then precipitated on ice and resuspended to adjust the condition suitable for binding genomic DNA. 
Extracted DNA is ready for downstream molecular-based applications including qPCR, 16S rRNA gene sequencing, etc.

MagCore® Gut Microbiome DNA Kit

Feature

Streamlined protocols for isolating inhibitor-free, PCR-quality DNA (up to 50 μg/prep) from microbes including Gram-positive and Gram-negative bacteria.

Hands-on time can be as little as 20 minutes.

Depletion of PCR inhibitors by precipitation using a novel one-step IR Buffer.

High-purity DNA is ready for common downstream applications such as qPCR and 16S rRNA gene sequencing.

Applications

The purified DNA can be directly used for downstream applications such as:

  1. qPCR
  2. 16S rRNA gene Sequencing
  3. Next-Generation Sequencing Applications

Performance

Stool DNA Extraction Efficiency


DNA was isolated from 0.2 g stool samples (in triplicates) with the MagCore Gut Microbiome DNA kit and supplier kits.
(supplier T, supplier Z, supplier Q1, supplier Q2).
DNA concentration and purity (A260/280, A260/230) are measured by Nanodrop spectrophotometer.

Gel electrophoresis

Analysis of Genomic DNA extracted from human stool samples using 1% gel electrophoresis.
Performance of MagCore Gut Microbiome kit analyzed on agarose gel electrophoresis; Lane M: RBC 1 kb DNA ladder Marker.

16S rRNA gene qPCR

DNA extracted from stool samples using MagCore 504 kit and other competitor kits was evaluated by 10-fold serial dilution of 16S rRNA gene qPCR amplification.
Non-template control (NTC) was included as a reference. (amplicon size: 466bp).

qPCR amplification curve of MagCore® 504, supplier Q2, supplier Z: MagCore 504 shows a low inhibition, 
similar amplification curve with supplier Q2, and superior Cq values to supplier Z.

Human fecal microbiota qPCR analysis

Phylum Target organism Gram Staining
Bacteroidetes Bacteroides–Prevotella–Porphyromonas G -
Actinobacteria Bifidobacterium G +
Actinobacteria Clostridium coccoides–Eubacterium rectale group G +
Firmicutes Enterococcus spp. G +

Human fecal microbiota qPCR analysis of four bacterial targets: Bacteroides–Prevotella–Porphyromonas, Bifidobacterium, Clostridium coccoides–Eubacterium
rectangle group, Enterococcus spp. DNA from triplicate stool samples was isolated with MagCore gut microbiome DNA kit and other suppliers.

Specification

Cartridge Contents

Cartridge Contents
  • Cartridge Contents
  • Cartridge Contents
  • Cartridge Contents
  • Cartridge Contents
  • Cartridge Contents
  • Cartridge Contents
  • Cartridge Contents
  • 1

    Heat block Well 1

  • 2

    Heat block Well 2

  • 3

    Empty

  • 4

    Empty

  • 5

    Elution Buffer 1000µl

  • 6

    ddH2O 1000µl

  • 7

    Wash 2 Buffer 1000µl

  • 8

    Wash 1 Buffer 1000µl

  • 9

    Beads Mixture 500μl

  • 10

    Binding Buffer 1000µl

  • 11

    Lysis Buffer 500µl

  • 12

    Empty

  • 13

    Magnetic Beads Separation Well 1

  • 14

    Magnetic Beads Separation Well 2

Cat.No. : MGM-01

introduction

Storage And Stability : Store at Room Temperature

Packing : 24 tests / Kit

Contents

Pre-Filled Cartridge Reagent ( 24 pcs )

Pipette Tip and Holder Set ( 24 sets )

Sample Tube ( 24 pcs )

Elution Tube ( 24 pcs )

Beads-Beating Tube ( 24 pcs )

GM-L1 Buffer ( 40ml/tube x 1 pcs )

GM-L2 Buffer ( 10ml/tube x 1 pcs )

GM-IR Buffer ( 20ml/tube x 1 pcs )

GM-P1 Buffer ( 10ml/tube x 1 pcs )

GM-P2 Buffer ( 15ml/tube x 1 pcs )

GM-LY Buffer ( 40ml/tube x 1 pcs )

Cat.No. : MGM-01

Cat.No. : MGM-02

introduction

Storage And Stability : Store at Room Temperature

Packing : 72 tests / Kit

Contents

Pre-Filled Cartridge Reagent ( 72 pcs )

Pipette Tip and Holder Set ( 72 sets )

Sample Tube ( 72 pcs )

Elution Tube ( 72 pcs )

Beads-Beating Tube ( 72 pcs )

GM-L1 Buffer ( 40ml/tube x 2 pcs )

GM-L2 Buffer ( 10ml/tube x 1 pcs )

GM-IR Buffer ( 20ml/tube x 2 pcs )

GM-P1 Buffer ( 10ml/tube x 1 pcs )

GM-P2 Buffer ( 15ml/tube x 2 pcs )

GM-LY Buffer ( 40ml/tube x 2 pcs )

Cat.No. : MGM-02

Back to List
關閉