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Home Products MagCore Kits Total RNA MagCore® Total RNA FFPE One-Step Kit
605
Cat.No.:
  • MRF-01
  • MRF-03

MagCore® Total RNA FFPE One-Step Kit

For extraction of total RNA from formalin-fixed paraffin-embedded (FFPE) tissue

MagCore® Total RNA FFPE One-Step Kit is specially designed for total RNA purification from FFPE tissues by MagCore® instruments. It features the method, of one-step heating, to melt paraffin without harmful reagents involved such as xylene or other organic solvents, and lyse tissues at the same time. The MagCore® Total RNA FFPE One-Step Kit System optimizes the lysis conditions to reverse the formalin fixation without the need for overnight digestion and retain both large and small RNAs. The program provides optional DNase I treatment to remove contaminated DNA.

MagCore® Total RNA FFPE One-Step Kit

Feature

A full package for total RNA isolation from FFPE tissue samples. It starts with melting paraffin to the final RNA purification.

Highly user-friendly protocol with minimal pretreatment required.

Sula oil (Deparaffinization solution): no xylene or other organic solvents involved.

The thermostable cap is specially designed to cover the reaction well for an optimal condition where the lysis buffer and the FFPE samples can mix properly during the long processing period.

The total processing time is approximately 160 minutes.

Applications

The purified RNA can be directly used for downstream applications such as:

  1. cDNA synthesis
  2. real-time PCR
  3. RT-PCR 
  4. Microarray
  5. Next-Generation Sequencing

FFPE RNA extraction efficiency

MagCore® 605 competitors

Performance

RNA integrity

  Competitor Q MagCore® 605
Tissue Block Sample RNA yields (ng/μl) RIN Sample RNA yields (ng/μl) RIN
#1 Q1 97.37 2.2 R1 77.9 3.2
#2 Q2 58.7 2.2 R2 62.6 3.3

RNA integrity assessment
Total RNA isolation from rat liver 50μm tissue sections of FFPE tissue blocks were measured on an Agilent 2100 bioanalyzer.
Isolated RNA from the MagCore® 605 kit maintains its integrity(18S, 28S peaks).
MagCore® 605 competitors

Brand Sample nanodrop
(ng/μl)		 A260/280 A260/230 Ct Value
(GAPDH)
MagCore®
605		 1 60.12 1.93 1.48 24.01
2 74.79 1.89 1.52 22.02
Supplier Z 3 31.21 1.96 1.49 26.61
Supplier Q 4 117.54 1.93 2.17 23.75
Supplier T 5 5.89 1.46 0.15 27.39
Comparison of total RNA extraction efficiency by using various FFPE RNA extraction kit suppliers
Total RNA was purified from one rat liver 50μm tissue section of a single FFPE tissue block for each sample.
The RNA yield and quality were quantified by Nanodrop n-1000.

 

RT-PCR performance

Evaluation of RNA performance in RT-PCR
Five amplicons of different lengths (118, 206, 312, 400, 503, 613 bp amplicon size) of the gene Rpl4 were performed using cDNA derived from FFPE rat liver samples purified by various suppliers.
 

Specification

Cartridge Contents

Cartridge Contents
  • Cartridge Contents
  • Cartridge Contents
  • Cartridge Contents
  • Cartridge Contents
  • Cartridge Contents
  • Cartridge Contents
  • Cartridge Contents
  • Cartridge Contents
  • 1

    Heat block Well 1

  • 2

    Heat block Well 2

  • 3

    Empty

  • 4

    Empty

  • 5

    RNase Free Water 1000µl

  • 6

    RNase Free Water 1000µl

  • 7

    Wash 2 Buffer 1000μl

  • 8

    Wash 1 Buffer 1000μl

  • 9

    Beads Mixture 500μl

  • 10

    Binding Buffer 1000µl

  • 11

    Lysis Buffer N 1000μl

  • 12

    Proteinase K 50µl

  • 13

    Magnetic Beads Separation Well 1

  • 14

    Magnetic Beads Separation Well 2

Cat.No. : MRF-01

introduction

Storage And Stability : Store at Room Temperature

Packing : 24 tests / Kit

Contents

Pre-Filled Cartridge Reagent ( 24 pcs )

Pipette Tip and Holder Set ( 48 sets )

Elution Tube ( 24 pcs )

Sula Oil ( 25ml/tube x 1 pcs )

Thermostable Cap ( 24 pcs )

Cat.No. : MRF-01

Cat.No. : MRF-03

introduction

Storage And Stability : Store at Room Temperature

Packing : 72 tests / Kit

Contents

Pre-Filled Cartridge Reagent ( 72 pcs )

Pipette Tip and Holder Set ( 144 sets )

Elution Tube ( 72 pcs )

Sula Oil ( 50ml/tube x 1 pcs )

Thermostable Cap ( 72 pcs )

Cat.No. : MRF-03

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