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Home Products MagCore Kits Total RNA MagCore® mRNA One-Step Kit
639
Cat.No.:
  • MRM-01
  • MRM-03

MagCore® mRNA One-Step Kit

For extraction of poly(A)-mRNA from human whole blood, culture animal cell, total RNA

Applicable Models : EDAPlus IISuperPlus II Dx

MagCore® mRNA One-Step Kit is specially designed for poly(A)-mRNA purification from human whole blood/ culture animal cell / total RNA. The program provides optional DNase I treatment to remove residual DNA from contaminating the results. High-quality DNA-free mRNA can be extracted using this kit along with our RNase-free DNase I. The isolation protocol relies on base pairing between the polyA residues at the 3’end of most eukaryote mRNA, and the oligo (dT)25 residues covalently coupled to the surface of the beads. Other RNA species lacking a polyA tail will not hybridize with the beads and are readily washed away.

MagCore® mRNA One-Step Kit

Feature

Exclude the RNA species which lacks a polyA tail.

Extract High-quality DNA-free mRNA by combining it with our RNase-free DNase I

Optional DNase I treatment to remove residual DNA.

Applications

Purified poly(A)-mRNA can be directly used for downstream applications such as:

  1. Real-time RT-PCR
  2. RT-PCR
  3. cDNA synthesis

Performance

Blood mRNA extraction efficiency

  Kit 400ul pre-treatment Blood Elution
buffer
without DNase with DNase
Magcore
639		 D N Magcore
639		 D N
mRNA
100nt		 Average 22.10 21.53 22.12 22.32 21.83 25.05 N/A
SD 0.14 0.26 0.26 0.21 0.48 0.08
CV 0.6% 1.2% 1.2% 0.9% 2.2% 0.3%
mRNA
500nt		 Average 22.86 22.05 22.95 23.10 22.54 27.06 N/A
SD 0.33 0.89 0.43 0.24 0.53 1.58
CV 1.3% 4.0% 1.9% 1.0% 2.3% 5.8%
DNA
100bp		 Average 30.10 36.41 27.47 38.72 36.86 38.21 N/A
SD 0.91 0.36 0.48 1.43 - 0.23
CV 3.0% 1.0% 1.7% 3.7% 0.6%


Cultured cell mRNA extraction efficiency

  Kit 1*10^6 THP-1 cell Elution
buffer
without DNase with DNase
Magcore
639		 D N Magcore
639		 D N
mRNA
100nt		 Average 17.18 17.37 16.48 17.1 17.18 17.17 N/A
SD 0.62 0.35 0.02 0.2 0.18 0.19
CV 3.60% 2.00% 0.10% 1.20% 1.00% 1.10%
mRNA
500nt		 Average 19.59 19.46 19.46 20 19.48 19.4 N/A
SD 0.34 0.54 0.29 0.08 0.18 0.21
CV 1.70% 2.70% 1.50% 0.40% 0.90% 1.10%
DNA
100bp		 Average 32.15 34.85 26.48 36.96 37.8 40 N/A
SD 0.5 1.56 0.42 - - -
CV 1.50% 4.50% 1.60%

Efficient removal of ribosomal RNA

L: Ladder
1: MagCore 639 mRNA from whole blood
2: MagCore 639 mRNA from pre-treatment blood
3: D brand kit mRNA from pre-treatment blood
4: N brand kit mRNA from pre-treatment blood
5: MagCore 639 mRNA from THP-1 cell
6: D brand kit mRNA from THP-1 cell
7: N brand kit mRNA from THP-1 cell

　
  Kit brand Sample Automatic/ Manual rRNA contamination
(%)
1 Magcore639 Whole blood Automatic 0
2 Magcore 639 Pre-treatment blood Automatic 0
3 D Manual 6
4 N 0
5 Magcore 639 THP-1 cell Automatic 2.6
6 D Manual 6.9
7 N 2.8

Specification

Cartridge Contents

Cartridge Contents
  • Cartridge Contents
  • Cartridge Contents
  • Cartridge Contents
  • Cartridge Contents
  • Cartridge Contents
  • Cartridge Contents
  • 1

    Heat block Well 1

  • 2

    Heat block Well 2

  • 3

    Empty

  • 4

    Empty

  • 5

    DNasel buffer 500µl

  • 6

    Elution buffer 500µl

  • 7

    Wash B Buffer 1200µl

  • 8

    Wash A Buffer 1200µl

  • 9

    Empty

  • 10

    Binding Buffer 500µl

  • 11

    Lysis Buffer 1200µl

  • 12

    Empty

  • 13

    Magnetic Beads Separation Well 1

  • 14

    Magnetic Beads Separation Well 2

Cat.No. : MRM-01

introduction

Storage And Stability : Store at Room Temperature

Packing : 24 tests / Kit

Contents

Pre-Filled Cartridge Reagent ( 24 pcs )

Pipette Tip and Holder Set ( 24 sets )

Sample Tube ( 24 pcs )

Elution Tube ( 24 pcs )

RBC Lysis Buffer ( 200ml/tube x 1 pcs )

Lysis buffer (mRNA) ( 10ml/tube x 1 pcs )

mRNA beads Mixture ( 13ml/tube x 1 pcs )

Cat.No. : MRM-03

introduction

Storage And Stability : Store at Room Temperature

Packing : 72 tests / Kit

Contents

Pre-Filled Cartridge Reagent ( 72 pcs )

Pipette Tip and Holder Set ( 72 sets )

Sample Tube ( 72 pcs )

Elution Tube ( 72 pcs )

Lysis buffer (mRNA) ( 30ml/tube x 1 pcs )

RBC Lysis Buffer ( 200ml/tube x 2 pcs )

mRNA beads Mixture ( 13ml/tube x 3 pcs )

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