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701

Cat.No.:

MNC-01
MNC-02

MagCore® NGS Auto Size-Select purification Kit

For selective DNA purification and size selection for NGS

Applicable Models : EDA Plus II Super HF16 HF16 Plus Compact

MagCore® NGS Auto Size-Select purification Kit is designed for fast DNA fragment cleanup and size selection. The cleanup program results in 80% or higher recovery while removing all adapter dimers in sample that is then applicable during the preparation of Next Generation Sequencing (NGS) library. For DNA fragment size selection, the program selectively purifies nucleic acid fragments from 100-200 bp or from 200-500 bp in just minutes which is also suitable for library preparation. The entire purification procedure is fully automated and can be performed in 35 minutes.

Feature
Applications
Performance
Specification

Feature

Automated DNA Cleanup and Size Selection ~ 30min.

Precise Size Range Selection.

High Recovery Rate.

Easy and Rapid Workflow.

Consistent and Reliable.

Applications

The MagCore® NGS Auto Size-Select purification Kit is designed to purify DNA for various downstream applications, including:

DNA library peparation for NGS
PCR or real-time PCR

Performance

Product Introduction and Average Recovery

Program	Program Description	Avarage Recovery(%)
701A	DNA Cleanup	>80%
701B	DNA Size Selection 200-500bp	40~60%
701C	DNA Size Selection 100-200bp	>50%

To meet the requirement of NGS,
it is better to produce a NGS library with DNA fragment sizes between 100~200 bp
(https://doi.org/10.1016/B978-0-12-418687-3.00015-X)
or 200~500 bp
(https://doi.org/10.1007/s11262-018-1568-x).

Human genomic library prepared with DNA Library Prep Kit was analyzed
by an Agilent Technologies 2100 Bioanalyzer

Red Line: Before DNA Clean-up
Blue Line: After DNA Clean-up

Comparison of DNA Cleanup methods

M: 100bp Marker.
Lane1, 4, 7: MagCore NGS Auto Size-Select purification Kit
cleanup DNA sample with 701A program.
Lane 2, 5, 8: Brand A manually cleanup DNA sample.
Lane 3, 6, 9: Brand V manually cleanup DNA sample.

MagCore NGS Auto Size-Select purification Kit: results of the automatic cleanup of DNA sample
are comparable with Brand A and Brand V manual kits, resulting in high purity product with >80% recovery.

Precise Size Selection

M: 100bp Marker.
Lane 1: MagCore NGS Auto Size-Select purification Kit
size select 200-500bp with 701B program.
Lane 2: Brand A manually size select 200-500bp.
Lane 3: Brand V manually size select 200-500bp.

MagCore NGS Auto Size-Select purification Kit is completely aotomated and can effectively size select DNA from 200-500bp,
while Brand A and Brand V contain larger DNA fragments in the final product.

Specification

Cartridge Contents