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Home Products MagCore Kits Genomic DNA MagCore® Plasma DNA Extraction Kit 1.2 ml
105
Cat.No.:
  • MPD-1200

MagCore® Plasma DNA Extraction Kit 1.2 ml

For extraction of free circulating DNA from human plasma or serum

MagCore®  Plasma DNA Extraction Kit is designed for purifying free circulating DNA from human serum or plasma using MagCore®  automated extraction systems. The kit contains all required reagents and labware for automated purification based on magnetic-particle technology. Reagents necessary for a complete process are supplied and pre-filled in the cartridges, which can be easily loaded into the MagCore® instrument.

MagCore® Plasma DNA Extraction Kit 1.2 ml

Feature

High performance of purified DNA in downstream applications such as qPCR.

Efficient recovery of fragmented DNA.

Pre-filled and sealed buffer cartridges prevent contamination.

No phenol or chloroform extraction.

Efficient removal of contaminants and inhibitors.

Applications

The MagCore® Plasma DNA Extraction Kit efficiently purifies free circulating DNA.
Sample types include:

  1. Human plasma
  2. Human serum

High quality DNA available for downstream applications, including:

  1. PCR and real-time PCR
  2. Next Generation Sequencing (NGS)

Performance

HBV detection by Real-Time PCR
The isolations of HBV nucleic acid from samples containing different concentrations of HBV were subsequently detected by Real-Time PCR

Sensitivity test for HBV detection

Lane 1 1x100 HBV serums.
Lane 2 1x101 HBV serums.
Lane 3 1x102 HBV serums.
Lane 4 1x103 HBV serums.
Lane N Negative control.
Lane M RBC 100 bp DNA Ladder Marker.



Figure 1. Nested PCR results of HBV at different concentrations.
Viral nucleic acids were purified from samples containing different amount of HBV using MagCore® Viral Nucleic Acid Extraction Kit.

Sensitivity test for HCV detection

Lane 1 5x100 HCV serums.
Lane 2 5x101 HCV serums.
Lane 3 5x102 HCV serums.
Lane 4 5x103 HCV serums.
Lane 5 5x104 HCV serums.
Lane 6 5x105 HCV serums.
Lane P Positive control.
Lane N Negative control.
Lane M RBC 100 bp DNA Ladder Marker.


Figure 2. Nested PCR results of HCV at different concentrations.
Viral nucleic acids were purified from samples containing different amount of HCV using MagCore® Viral Nucleic Acid Extraction Kit.

Specification

Cartridge Contents

Cartridge Contents
  • Cartridge Contents
  • Cartridge Contents
  • Cartridge Contents
  • Cartridge Contents
  • Cartridge Contents
  • Cartridge Contents
  • Cartridge Contents
  • 1

    Heat block Well 1

  • 2

    Heat block Well 2

  • 3

    Empty

  • 4

    Empty

  • 5

    RNase Free Water 1000µl

  • 6

    RNase Free Water 1000µl

  • 7

    Wash 2 Buffer 1000μl

  • 8

    Wash 1 Buffer 1000μl

  • 9

    Beads Mixture 500μl

  • 10

    Binding Buffer 1500µl

  • 11

    Lysis Buffer 1200µl

  • 12

    Empty

  • 13

    Magnetic Beads Separation Well 1

  • 14

    Magnetic Beads Separation Well 2

Cat.No. : MPD-1200

introduction

Storage And Stability : Store at Room Temperature

Packing : 96 tests / Kit

Contents

Pre-Filled Cartridge Reagent ( 96 pcs )

Pipette Tip and Holder Set ( 96 sets )

Sample Tube ( 96 pcs )

Elution Tube ( 96 pcs )

Proteinase K ( 11 mg/tube x 2 pcs )

PK Storage Buffer ( 1.25 ml/tube x 2 pcs )

Cat.No. : MPD-1200

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