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Cat.No.:

MVN-1200

MagCore® Viral Nucleic Acid Large Volume Extraction Kit 1.2 ml

For extraction of viral DNA/RNA from serum plasma and cell-free body fluids

Applicable Models : EDA Plus II Super HF16 HF16 Plus HF48 Compact

The MagCore® Viral Nucleic Acid Extraction Kit is designed for purifying viral DNA and RNA from serum, plasma, and cell-free body fluids.MagCore® magnetic particle technology delivers high-quality DNA/RNA that is suitable for direct use in downstream applications such as amplification or other enzymatic reactions. To minimize the risk of cross-contamination, plastic consumables inside the Kit are DNase/RNase treated and the operation system is designed to individually process samples at the same time. Multiple protocols are installed on the instrument and optimized for different sample volumes.

MagCore® Viral Nucleic Acid Large Volume Extraction Kit 1.2 ml

Feature
Applications
Performance
Specification

Feature

High performance of purified DNA in downstream applications such as qPCR.

High analytical sensitivity.

Cartridges are pre-filled and sealed to prevent contamination.

Walkaway processing improves work efficiency.

Applications

MagCore® Viral Nucleic Acid Extraction Kit is designed to allow automated processing of multiple sample types in the same run.
Sample types include:

Cell-free body fluids
Plasma and serum
CSF
Urine

Performance

HBV detection by Real-Time PCR
The isolations of HBV nucleic acid from samples containing different concentrations of HBV were subsequently detected by Real-Time PCR

Sensitivity test for HBV detection

Lane 1	1x100 HBV serums.
Lane 2	1x101 HBV serums.
Lane 3	1x102 HBV serums.
Lane 4	1x103 HBV serums.
Lane N	Negative control.
Lane M	RBC 100 bp DNA Ladder Marker.

Figure 1. Nested PCR results of HBV at different concentrations.
Viral nucleic acids were purified from samples containing different amounts of HBV using MagCore® Viral Nucleic Acid Extraction Kit.

Sensitivity test for HCV detection

Lane 1	5x100 HCV serums.
Lane 2	5x101 HCV serums.
Lane 3	5x102 HCV serums.
Lane 4	5x103 HCV serums.
Lane 5	5x104 HCV serums.
Lane 6	5x105 HCV serums.
Lane P	Positive control.
Lane N	Negative control.
Lane M	RBC 100 bp DNA Ladder Marker.

Figure 2. Nested PCR results of HCV at different concentrations.
Viral nucleic acids were purified from samples containing different amount of HCV using MagCore® Viral Nucleic Acid Extraction Kit.

Specification

Cartridge Contents

1
Heat block Well 1
2
Heat block Well 2
3
Empty
4
Empty
5
RNase Free Water 1000µl
6
RNase Free Water 1000µl
7
Wash 2 Buffer 1500µl
8
Wash 1 Buffer 1500µl
9
Beads Mixture 500μl
10
Binding Buffer 1500µl
11
Lysis Buffer 1200µl
12
Empty
13
Magnetic Beads Separation Well 1
14
Magnetic Beads Separation Well 2

Cat.No. : MVN-1200

introduction

Storage And Stability : Store at Room Temperature

Packing : 96 tests / Kit

Contents

Pre-Filled Cartridge Reagent ( 96 pcs )

Pipette Tip and Holder Set ( 96 sets )

Sample Tube ( 96 pcs )

Elution Tube ( 96 pcs )

Carrier RNA ( 1 mg/pcs x 1 pcs )

RNase Free Water ( 1.25 ml/tube x 1 pcs )

Proteinase K ( 11 mg/tube x 2 pcs )

PK Storage Buffer ( 1.25 ml/tube x 2 pcs )

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