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Home Products MagCore Kits Genomic DNA MagCore® Genomic DNA FFPE One-Step Kit
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Cat.No.:
  • MGF-01
  • MGF-03

MagCore® Genomic DNA FFPE One-Step Kit

For extraction of total DNA from formalin-fixed paraffin-embedded FFPE tissue

MagCore® Genomic DNA FFPE One-Step Kit is designed to purify total DNA (including genomic, mitochondrial, and viral DNA) from Formalin-fixed Paraffin-embedded tissues via MagCore® Automated Extraction instruments. Our program features One-Step Heating, which automatically melts paraffin and lyses cells at the same time. No harmful reagent such as xylene is involved in the deparaffination process. Two protocols are outlined for different tissues sizes: 2-hour setup for smaller tissues and 16-hour setup for larger tissues.

MagCore® Genomic DNA FFPE One-Step Kit

Feature

Can load FFPE tissue samples with a surface area of up to 300 mm2 and up to 5µm thick.

A full package for DNA to be purified from FFPE samples. Complete automatic procedure from deparaffination to elution.

Stable deparaffination by Sula oil and reproducible results; no xylene involved.

Thermostavle cap is specially designed to cover the reaction well for an optimal condition where the lysis buffer and the FFPE sample can mix properly during the processing period.

Applications

  1. PCR and real-time quantitative PCR
  2. Southern Blot
  3. Next Generation Sequencing
  4. Genotyping

Performance

2-hr heating protocol works out
There are 2-hour heating and 16-hour heating protocols.
It is suggested for a better performance that the 2-hour heating program is for small tissues and the 16-hour program is for large tissues.

Genomic DNA with high performance
The DNA extracted by MagCore® Genomic DNA FFPE One-Step Kit is high yield and suitable for downstream PCR and real-time PCR.

A. DNA concentration
Whether samples were treated with either a 2-hour or 16-hour heating, yields of DNA extracted by MagCore® are mostly higher than the yields of DNA extracted by the competitor.


B. The downstream PCR
PCR products of DNA purified by MagCore® and the competitor brand are confirmed by 1.5% agarose gel electrophoresis.

C. Purified DNA ideal for the downstream real-time PCR
Whether samples experience a 2-hour or 16-hour heating,
the CT values of samples processed by MagCore® are mostly lower than the CT values of samples processed by the competitor.

 
 
D. Tissue sample sizes
It is shown below that different sizes of tissue samples were processed by a 2-hour or 16-hour heating program.
2-hour heating
  Sample Size(cm2)
1 319 1*1
2 317 0.8*0.8
3 170 0.5*1
4 171 1.5*1
5 354 1.5*2
6 349 1.5*2
7 207 0.5*0.5
8 208 0.5*1
9 209 0.5*1
16-hour heating
  Sample Size(cm2)
1 128 2*3
2 191 2*2
3 350 2*2
4 362 2*3
5 369 2*2
6 304 2*3
7 206 1.5*1
8 207 0.5*0.5
9 208 0.5*1
10 209 1*1
DNA yield gained from 10 mg of mice tissue samples and 0.25 cm tail

Sample ID Yield (ng/ul)
Liver 17.5
Spleen 17.5
Brain 10
Lung 12.5
Kidney 32.5
Tail 7.5

Specification

Cartridge Contents

Cartridge Contents
  • Cartridge Contents
  • Cartridge Contents
  • Cartridge Contents
  • Cartridge Contents
  • Cartridge Contents
  • Cartridge Contents
  • Cartridge Contents
  • Cartridge Contents
  • 1

    Heat block Well 1

  • 2

    Heat block Well 2

  • 3

    Empty

  • 4

    Empty

  • 5

    Elution Buffer 1000µl

  • 6

    ddH2O 1000µl

  • 7

    Wash 2 Buffer 1000µl

  • 8

    Wash 1 Buffer 1000µl

  • 9

    Beads Mixture 500μl

  • 10

    Binding Buffer 1000µl

  • 11

    Lysis Buffer 500µl

  • 12

    GT buffer 500µl

  • 13

    Magnetic Beads Separation Well 1

  • 14

    Magnetic Beads Separation Well 2

Cat.No. : MGF-01

introduction

Storage And Stability : Store at Room Temperature

Packing : 36 tests / Kit

Contents

Pre-Filled Cartridge Reagent ( 36 pcs )

Pipette Tip and Holder Set ( 72 sets )

Elution Tube ( 36 pcs )

Sula Oil ( 25ml/tube x 1 pcs )

Proteinase K ( 11 mg/tube x 1 pcs )

PK Storage Buffer ( 1.25 ml/tube x 1 pcs )

Thermostable Cap ( 36 pcs )

Cat.No. : MGF-01

Cat.No. : MGF-03

introduction

Storage And Stability : Store at Room Temperature

Packing : 72 tests / Kit

Contents

Pre-Filled Cartridge Reagent ( 72 pcs )

Pipette Tip and Holder Set ( 144 sets )

Elution Tube ( 72 pcs )

Sula Oil ( 50ml/tube x 1 pcs )

Proteinase K ( 11 mg/tube x 2 pcs )

PK Storage Buffer ( 1.25 ml/tube x 2 pcs )

Thermostable Cap ( 72 pcs )

Cat.No. : MGF-03

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