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Home Products MagCore Kits Genomic DNA MagCore® Gut Microbiome DNA Kit
504
Cat.No.:
  • MGM-01
  • MGM-02

MagCore® Gut Microbiome DNA Kit

For extraction of microbial and host genomic DNA from stool samples

MagCore® Gut Microbiome DNA Kit is designed specifically for isolating high-quality microbial and host genomic DNA from stool and gut samples. With a short pretreatment, DNA from tough-to-lyse Gram-positive and Gram-negative bacteria which inhabit fecal samples can be successfully isolated with efficient lysis and homogenized with Beads-Beating Tube. In addition, inhibitor substances (e.g. polysaccharides, protein, etc.) commonly found in stools are removed with one-step IR Buffer. The crude DNA is then precipitated on ice and resuspended to adjust the condition that is suitable for binding genomic DNA. 
Extracted DNA is ready for downstream molecular-based applications including qPCR, 16S rRNA gene sequencing, etc.

MagCore® Gut Microbiome DNA Kit

Feature

Streamlined protocols for the isolation of inhibitor-free, PCR-quality DNA (up to 50 μg/prep) from microbes including Gram-positive and Gram-negative bacteria.

Hands-on time can be as little as 20 minutes.

Depletion of PCR inhibitors by precipitation using a novel one-step IR Buffer.

High-purity DNA is ready for common downstream applications such as qPCR and 16S rRNA gene sequencing.

Applications

The purified DNA can be directly used for downstream application such as:

  1. qPCR
  2. 16S rRNA gene Sequencing
  3. Next-Generation Sequencing Applications

Performance

Stool DNA Extraction Efficiency


DNA was isolated from 0.2 g stool samples (in triplicates) with the MagCore Gut Microbiome DNA kit and supplier kits.
(supplier T, supplier Z, supplier Q1, supplier Q2).
DNA concentration and purity (A260/280, A260/230) are measured by Nanodrop spectrophotometer.

Gel electrophoresis

Analysis of Genomic DNA extracted from human stool samples using 1% gel electrophoresis.
Performance of MagCore Gut Microbiome kit analyzed on agarose gel electrophoresis; Lane M: RBC 1 kb DNA ladder Marker.

16S rRNA gene qPCR

DNA extracted from stool samples using MagCore 504 kit and other competitor kits were evaluated by 10-fold serial dilution of 16S rRNA gene qPCR amplification.
Non template control (NTC) was included as a reference. (amplicon size: 466bp).

qPCR amplification curve of MagCore® 504, supplier Q2, supplier Z: MagCore 504 shows a low inhibition, 
similar amplification curve with supplier Q2, and superior Cq values to supplier Z.

Human fecal microbiota qPCR analysis

Phylum Target organism Gram Staining
Bacteroidetes Bacteroides–Prevotella–Porphyromonas G -
Actinobacteria Bifidobacterium G +
Actinobacteria Clostridium coccoides–Eubacterium rectale group G +
Firmicutes Enterococcus spp. G +

Human fecal microbiota qPCR analysis of four bacterial targets: Bacteroides–Prevotella–Porphyromonas, Bifidobacterium, Clostridium coccoides–Eubacterium
rectale group, Enterococcus spp.. DNA from triplicate stool samples were isolated wih MagCore gut microbiome DNA kit and other suppliers.
 

Specification

Cartridge Contents

Cartridge Contents
  • Cartridge Contents
  • Cartridge Contents
  • Cartridge Contents
  • Cartridge Contents
  • Cartridge Contents
  • Cartridge Contents
  • Cartridge Contents
  • 1

    Heat block Well 1

  • 2

    Heat block Well 2

  • 3

    Empty

  • 4

    Empty

  • 5

    Elution Buffer 1000µl

  • 6

    ddH2O 1000µl

  • 7

    Wash 2 Buffer 1000µl

  • 8

    Wash 1 Buffer 1000µl

  • 9

    Beads Mixture 500μl

  • 10

    Binding Buffer 1000µl

  • 11

    Lysis Buffer 500µl

  • 12

    Empty

  • 13

    Magnetic Beads Separation Well 1

  • 14

    Magnetic Beads Separation Well 2

Cat.No. : MGM-01

introduction

Storage And Stability : Store at Room Temperature

Packing : 24 tests / Kit

Contents

Pre-Filled Cartridge Reagent ( 24 pcs )

Pipette Tip and Holder Set ( 24 sets )

Sample Tube ( 24 pcs )

Elution Tube ( 24 pcs )

Beads-Beating Tube ( 24 pcs )

GM-L1 Buffer ( 40ml/tube x 1 pcs )

GM-L2 Buffer ( 10ml/tube x 1 pcs )

GM-IR Buffer ( 20ml/tube x 1 pcs )

GM-P1 Buffer ( 10ml/tube x 1 pcs )

GM-P2 Buffer ( 15ml/tube x 1 pcs )

GM-LY Buffer ( 40ml/tube x 1 pcs )

Cat.No. : MGM-01

Cat.No. : MGM-02

introduction

Storage And Stability : Store at Room Temperature

Packing : 72 tests / Kit

Contents

Pre-Filled Cartridge Reagent ( 72 pcs )

Pipette Tip and Holder Set ( 72 sets )

Sample Tube ( 72 pcs )

Elution Tube ( 72 pcs )

Beads-Beating Tube ( 72 pcs )

GM-L1 Buffer ( 40ml/tube x 2 pcs )

GM-L2 Buffer ( 10ml/tube x 1 pcs )

GM-IR Buffer ( 20ml/tube x 2 pcs )

GM-P1 Buffer ( 10ml/tube x 1 pcs )

GM-P2 Buffer ( 15ml/tube x 2 pcs )

GM-LY Buffer ( 40ml/tube x 2 pcs )

Cat.No. : MGM-02

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