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Home Products MagCore Kits Total RNA MagCore® triXact RNA Kit
631
Cat.No.:
  • MRX-01
  • MRX-03

MagCore® triXact RNA Kit

For extraction of total RNA from cultured cells, whole blood and tissues

Applicable Models : EDAPlus IISuperHF16HF16 Plus

MagCore® triXact RNA Kit is specially designed for total RNA purification from the three most common sample types in diagnostics, research, and forensics: up to 1x10⁶ cultured cells, a variety of tissues, or whole blood samples. The program provides optional DNase I treatment to remove residual DNA and extract high quality DNA-free RNA. Make use of its oustanding extraction performance, effiency, plus easy-to-follow protocols to simplify your every day extraction!

MagCore® triXact RNA Kit

Feature

Excellent RNA Yield and Purity shown in both A260/A280 ratio and RIN value.

Highly efficienct and user-friendly protocol with minimal pretreatment required.

Capable of isolating RNA from 3 types of samples: Cells, Whole blood, Tissues.

Optional DNase I treatment to remove residual DNA.

Applications

Purified RNA ready to use in downstream applications such as: 

  1. PCR
  2. Real-time PCR
  3. Microarray target preparation
  4. Northern blotting
  5. NGS analysis

Performance

Total RNA from 1 x 106 Hela Cell Extract
No. Kit nanodrop
(ng/µl)		 260/280 260/230
1 MagCore®
triXact RNA Kit		 264.69 2.04 2.11
2 250.61 2.02 2.17
3 237.44 2.03 2.17
4 Brand Q 207.15 2.08 1.86
5 182.85 2.07 1.78
6 174.9 2.11 1.63
7 Brand Z 171.52 2.03 2.24
8 210.45 2.02 2.22
9 164.76 2.05 2.17
10 Brand M 224.35 2.11 2.26
11 252.69 2.10 2.3
12 222.45 2.08 2.18
Table 1. Analysis of Cell RNA from MagCore® triXact Kit and others
　

Fig 1. Gel Electrophoresis of RNA from 1 x 106 Hela Cell Extract
Total RNA from 400μl Human Whole Blood

Fig 3. Concentration comparison of RNA from 400μl Human Whole Blood.
　
No. Kit nanodrop
(ng/µl)		 260/280 260/230
1 MagCore®
triXact RNA Kit		 96.87 1.94 1.18
2 106.00 1.92 2.02
3 102.06 1.95 2.06
4 Brand Q 28.77 1.99 1.69
5 20.88 2.06 0.31
6 15.02 1.89 0.85
7 Brand Z 21.36 1.97 1.82
8 23.32 1.98 1.48
9 25.5 1.71 1.26
10 Brand M 27.09 1.91 0.73
11 22.09 2.35 1.23
12 30.13 2.08 0.37

Table 2. Analysis of Whole Blood RNA isolated by MagCore® triXact RNA Kit and others.
　
No. Kit whole blood
(ml)		 nanodrop
(ng/µl)		 260/280 260/230
1 EDTA 0.4 40.08 2.03 1.69
2 0.8 189.10 1.96 2.09
3 1.2 256.82 1.94 2.12
4 Lithium Heparin 0.4 62.19 1.98 1.79
5 0.8 137.97 1.96 1.98
6 1.2 132.37 1.96 1.96
7 Sodium Citrate
3.2%		 0.4 84.52 1.93 1.77
8 0.8 148.96 1.94 2.01
9 1.2 240.19 1.94 2.15
10 BD
Vacutainer ACD
Solution A		 0.4 75.06 1.94 1.79
11 0.8 134.10 1.95 1.93
12 1.2 176.26 1.95 1.16

Table 3. Extraction efficiency of RNA from Human Whole Blood with various anticoagulants.
Total RNA from 30 mg Fish Tissue

Fig 4. Concentration comparison of RNA from 30 mg Fish Tissue with/without DNase I treatment
　

Specification

Cartridge Contents

Cartridge Contents
  • Cartridge Contents
  • Cartridge Contents
  • Cartridge Contents
  • Cartridge Contents
  • Cartridge Contents
  • Cartridge Contents
  • Cartridge Contents
  • 1

    Heat block Well 1

  • 2

    Heat block Well 2

  • 3

    Empty

  • 4

    Empty

  • 5

    RNase Free Water 1000µl

  • 6

    RNase Free Water 1000µl

  • 7

    Wash 2 Buffer 1000μl

  • 8

    Wash 1 Buffer 1000μl

  • 9

    Beads Mixture 500μl

  • 10

    Binding Buffer 1000µl

  • 11

    Lysis Buffer 500µl

  • 12

    Empty

  • 13

    Magnetic Beads Separation Well 1

  • 14

    Magnetic Beads Separation Well 2

Cat.No. : MRX-01

introduction

Storage And Stability : Store at Room Temperature

Packing : 24 tests / Kit

Contents

Pre-Filled Cartridge Reagent ( 24 pcs )

Pipette Tip and Holder Set ( 24 sets )

Sample Tube ( 24 pcs )

Elution Tube ( 24 pcs )

RBC Lysis Buffer ( 100ml/tube x 1 pcs )

RB Buffer ( 30ml/tube x 1 pcs )

Filter Column Set ( 25 pcs )

Cat.No. : MRX-03

introduction

Storage And Stability : Store at Room Temperature

Packing : 72 tests / Kit

Contents

Pre-Filled Cartridge Reagent ( 72 pcs )

Pipette Tip and Holder Set ( 72 sets )

Sample Tube ( 72 pcs )

Elution Tube ( 72 pcs )

RBC Lysis Buffer ( 200ml/tube x 1 pcs )

RB Buffer ( 60ml/tube x 1 pcs )

Filter Column Set ( 75 pcs )

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