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Cat.No.:

MRM-01
MRM-03

MagCore® mRNA One-Step Kit

For extraction of poly(A)-mRNA from human whole blood, culture animal cell, total RNA

Applicable Models : EDA Plus II Super Plus II Dx

MagCore® mRNA One-Step Kit is specially designed for poly(A)-mRNA purification from human whole blood/ culture animal cell / total RNA. The program provides optional DNase I treatment to remove residual DNA from contaminating the results. High quality DNA-free mRNA can be extracted using this kit along with our RNase-free DNase I. The isolation protocol relies on base pairing between the polyA residues at the 3’end of most eukaryote mRNA, and the oligo (dT)25 residues covalently coupled to the surface of the beads. Other RNA species lacking a polyA tail will not hybridize with the beads and are readily washed away.

Feature
Applications
Performance
Specification

Feature

Exclude the RNA species which lacks a polyA tail.

Extract High-quality DNA-free mRNA by combine with our RNase-free DNase I

Optional DNase I treatment to remove residual DNA.

Applications

Purified poly(A)-mRNA can be directly used for downstream application such as::

Real-time RT-PCR
RT-PCR
cDNA synthesis

Performance

Blood mRNA extraction efficiency

	Kit	400ul pre-treatment Blood						Elution buffer
		without DNase			with DNase
		Magcore 639	D	N	Magcore 639	D	N
mRNA 100nt	Average	22.10	21.53	22.12	22.32	21.83	25.05	N/A
	SD	0.14	0.26	0.26	0.21	0.48	0.08
	CV	0.6%	1.2%	1.2%	0.9%	2.2%	0.3%
mRNA 500nt	Average	22.86	22.05	22.95	23.1	22.54	27.06	N/A
	SD	0.33	0.89	0.43	0.24	0.53	1.58
	CV	1.3%	4.0%	1.9%	1.0%	2.3%	5.8%
DNA 100bp	Average	30.10	36.41	27.47	38.72	36.86	38.21	N/A
	SD	0.91	0.36	0.48	1.43	-	0.23
	CV	3.0%	1.0%	1.7%	3.7%		0.6%

Cultured cell mRNA extraction efficiency

	Kit	1*10^6 THP-1 cell						Elution buffer
		without DNase			with DNase
		Magcore 639	D	N	Magcore 639	D	N
mRNA 100nt	Average	17.18	17.37	16.48	17.1	17.18	17.17	N/A
	SD	0.62	0.35	0.02	0.2	0.18	0.19
	CV	3.6%	2.0%	0.1%	1.2%	1.0%	1.1%
mRNA 500nt	Average	19.59	19.46	19.46	20	19.48	19.4	N/A
	SD	0.34	0.54	0.29	0.08	0.18	0.21
	CV	1.7%	2.7%	1.5%	0.4%	0.9%	1.1%
DNA 100bp	Average	32.15	34.85	26.48	36.96	37.8	40	N/A
	SD	0.5	1.56	0.42	-	-	-
	CV	1.5%	4.5%	1.6%	-	-	-

Efficient removal of ribosomal RNA

L: Ladder
1: MagCore 639 mRNA from whole blood
2: MagCore 639 mRNA from pre-treatment blood
3: D brand kit mRNA from pre-treatment blood
4: N brand kit mRNA from pre-treatment blood
5: MagCore 639 mRNA from THP-1 cell
6: D brand kit mRNA from THP-1 cell
7: N brand kit mRNA from THP-1 cell

	Kit brand	Sample	Automatic/ Manual	rRNA contamination (%)
1	Magcore639	Whole blood	Automatic	0
2	Magcore 639	Pre-treatment blood	Automatic	0
3	D		Manual	6
4	N			0
5	Magcore 639	THP-1 cell	Automatic	260.0%
6	D		Manual	6.9
7	N			2.8